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KMID : 0616120190500010053
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Korean Journal of Pharmacognosy
2019 Volume.50 No. 1 p.53 ~ p.58
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Quantitative Analysis of Cyanidin-3-O-rutinoside and Quercetin-3-O-rutinoside from Extracts of Morus alba Fruit
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Kim Min-Ji
Lee Ji-Hyun
Pyo Jae-Sung
Kim Hye-Kyung
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Abstract
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Morus alba fruit contains several anthocyanins and flavonoids like cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside. Extractions from Morus alba fruit were performed with five different compositions of ethanol/water [v/v]. High-Performance Liquid Chromatography (HPLC) was used for the validation and content determination of cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside. The contents of cyanidin-3-O-rutinoside in ethanol extracts (100%, 70%, 40%, 20% and 0%, ethanol/water [v/v]) were 1.150%, 0.822%, 0.749%, 0.663% and 0.597%, respectively. Quercetin-3-O-rutinoside contents of ethanol extracts (100%, 70%, 40%, 20% and 0%, ethanol/water [v/v]) were 0.167%, 0.161%, 0.159%, 0.155% and 0.096%, respectively. The highest contents of cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside were in the ethanol extract with 100% ethanol/water [v/v]. These HPLC analysis method could be used as basic data for standardization of functional food from Morus alba fruit.
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KEYWORD
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Morus alba fruit, Cyanidin-3-O-rutinoside, Quercetin-3-O-rutinoside, Quantitative analysis, HPLC, Validation
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